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Pesticide Science

General Info Pesticide Science

The Laboratory of Pesticide Science of the Agricultural University of Athens (AUA) was founded in 1989 and it is one out of the eleven Laboratories of the Department of Crop Science of the AUA and one out of the five Laboratories of the Crop Protection and Environment Sector. The Laboratory is hosted at the fist floor of "Triantafillides" Building at the northeast side of the Campus (close to the main entrance) in an area of approximately 300 sq.m. and at the ground floor and the basement of the new building beside the arboretum (approx. 200 sq.m.).

The mission of the Laboratory of Pesticide Science is the teaching at undergraduate and post-graduate levels and the execution of research in a variety of modern topics of Pesticide Science and Crop Protection:

  • chemical crop protection
  • study of the mode(s)-of action (MoA) of pesticides
  • isolation, study, and identification of bioctive compounds of natural origin (natural products)
  • pesticide and mycotoxin residue analysis in food and the environment
  • study of the fate of pesticides in the environment
  • toxicological and eco-toxicological risk assessment of pesticides
  • biochemical, genetic, and molecular investigation of pest and pathogen resistance  to pesticides
  • development of metabolomics for the study of pesticides. 

The Laboratory is hosted in a modern and fully-equipped space, with infrastructure capable to support advance research. The chromatographic analytical facilities include a LC/MS system equipped with a quadrupole mass selective detector (MSD) configured for using ESI and APCI ionization modes; 3 HPLC systems equipped with fluorescence detector (FLD) and photo-diode array detector (DAD), 2 GC with a numbers of detectors (ECD, NPD, FID) and 1 GC/MS operated in electron impact ionization (EI) mode and two spectrophotometers UV/Vis. The biochemistry and molecular equipment include a MJ Research PTC200 thermal cycler with dual 48-well block, thermo horizontal electrophoresis gel systems, appropriate electrophoresis documentation and analysis system and an ultra low temperature laboratory Freezer (-86°C). There is also available an array of modern laboratory equipment including general purpose and orbital shaking and standing incubators for microbiological studies, shakers, solid-phase extraction manifolds, growth chambers for in vivo experiments, laboratory Freezer (-20°C), laminar airflow workbenches, autoclaves, rotary evaporators, water baths, sonicators, analytical balances, ovens, centrifuges, ultrapure water purification system and water distillation equipment. The laboratory facilities and equipment are important elements for a successful accomplishment of the major goals of the research project and will be provided to the project members, under the guidance and training of the experienced laboratory staff. Additional research and instructive work is provided in an experimental greenhouse for the establishment of in planta experiments.

Research interests: Pesticide effectiveness and biochemical mechanisms of pesticide activity. Biochemical, genetic and molecular research on the resistance of plant pathogens to fungicides. Pesticide resistance risk assessment of plant pathogens in the field. Development and implementation of molecular resistance diagnosis. Fungicide application and mycotoxin production by mycotoxigenic fungal species. Development and validation of analytical methods for mycotoxin determination in food and feed. Isolation and characterization of natural bioactive substances. Structure - effectiveness relations of bio-active substances. Use of micro-arrays for the identification of molecular targets and the development of pesticides. Development and validation of analytical methods for the determination of pesticide residues in agricultural products. Development of biotechnological methods for pesticide residues analysis. Environmental behavior of pesticides. Pesticide biodegradation. Pesticide in integrated pest management.

A large number of nationally and internationally-funded research projects has been executed by the Staff of the Laboratory. It collaborates with many Laboratories of the AUA, Laboratories of other research institutions in Greece (AUTH, NSCR Demokritos, Benaki Phytopathological Institute, University of Crete, University of Thessaly etc) and others abroad. Staff of the Laboratory participate in national and international conferences, they have published a vast number of research papers in international Journals, which have received significant recognition by the scientific community.

Also, the Laboratory participates and supports research activities of other labs of the AUA as well as those of other Universities and Institutions in Greece and abroad. 

+30 2105294546
+30 2105294514
vontas@aua.gr
+30 2105294543
+30 2105294514
bmic7fof@aua.gr
+30 2105294546
+30 null
konstantinos.aliferis@aua.gr
+30 2105294542
+30 2105294514
gpaziotou@aua.gr
+30 2105294546
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tasmal@aua.gr
Course name Credits Undergrad/Postgrad Compulsory/Elective
Special Topics of Pesticide Science Postgrad Elective
Pesticide Residues in the Food and the Environment Postgrad Elective
Pestiside Science 5 Undergrad Compulsory
Chemical Crop Protection 5 Undergrad Compulsory

Research Pesticide Science

 

Pesticide effectiveness and biochemical mechanisms of pesticise activity.

Biochemical, genetic and molecular research on the resistance of plant pathogens to fungicides.

Pesticide resistance risk assessment of plant pathogens in the field.

Development and implementation of molecular resistance diagnosis.

Insecticide resistance analysis of mosquitoes.

Fungicide application and mycotoxin production by mycotoxigenic fungal species.

Development and validation of analytical methods for mycotoxin determination in food and feed.

Isolation and characterization of natural bioactive substances.

Structure - effectiveness relations of bio-active substances.

Use of micro-arrays for the identification of molecular targets and the development of pesticides.

Development and validation of analytical methods for the determination of pesticide residues in agricultural products.

Development of biotechnological methods for pesticide residues analysis.

Environmental behavior of pesticides.

Pesticide biodegradation.

Pesticide in integrated pest management.

Effects of fungicides on wine-making yest.

Projects of Pesticide Science Laboratory

1993-1996: “Genetic control and mechanisms of resistance to fungicides affecting the sterol biosynthesis”, PENED 91/ΕΔ425, General Secretariat of Research and Technology.

1996-1998: “Strategies of plant defense against plant pathogens: The role of non-glandular leaf hairs in allelopathy”, PENED 95 /ΑΠ 1754, General Secretariat of Research and Technology.

1998-2000: “Studies on the genetic basis and biochemical mechanism of resistance to piperidine fungicides” Research project funded by State Scholarships Foundation.

2000-2001: “Antimicrobial activities of Dittrichia viscosa plant extracts” (GSRT-PENED 99 / ΕΔ 121).

2000-2001: “Phytopathological and molecular studies for the control of Botrytis cinerea using fungicides and yeasts”, PENED 99 / ΕΔ 188, General Secretariat of Research and Technology.

2000-2001: “Studies on the resistance risk assessment in natural compounds with antifungal properties”, Research project funded by Empeirikion Foundation.

2004-2007: “Genes and Pesticides: Development and application of molecular diagnostics for the determination of fungicide resistance”, PYTHAGORAS, General Secretariat of Research and Technology.

2004-2009: “Development of molecular diagnostics for the detection of fungicide resistance” Research project funded by Bayer CropScience Hellas, BASF Agro Hellas and Syngenta Hellas.

2005-2009: “Phytopathological and molecular studies for the determination of resistance to complex III inhibitors and development of molecular diagnostics”, PENED 03/ΕΔ171, Bayer CropScience Hellas, BASF Agro Hellas, General Secretariat of Research and Technology.

2005-2009: “Fungicides and Mycotoxins: Phytopathological and molecular characterization of fungicide resistance mutations and the role on mycotoxin production in Aspergillus and Fusarium species”, PYTHAGORAS II, General Secretariat of Research and Technology.

2005-2010: “Genetic and biochemical identification of natural antimicrobial metabolites produced by antagonistic bacteria”, PENED 03/ΕΔ230, General Secretariat of Research and Technology.

2008-2010: “Studies of endosulfan residues in olive oil”, Research project funded by Ministry of Rural Development and Food.

2010: “Study on the effect of insecticides in cellular population having genetics and membranes modification for use in biosensors”, Postgraduate research project funded by the Ministry of Education and Religions, HRAKLEITOS II, EPEAEK, ESPA 2007-2013.

2010: “Study on the effect of herbicides on non-target photosynthetic organisms by the use of bio-indicators, Postgraduate research project funded by the Ministry of Education and Religions, HRAKLEITOS II, EPEAEK, ESPA 2007-2013.

2011: “Strategy of managing the aflatoxin problem in dried figs within the framework of the integrated management of fig tree cultivation”. Acronym: AFLAFIG, ARCHIMIDIS, ID: teikal11, Ministry of Education and Religions, ESPA 2007-2013.

2011: Development of IT and molecular diagnostic tools, for improving the sustainability of pesticide based approaches to control agricultural pests of major economic importance in Greece”. Acronym: GR-RBase, THALIS, ID: 426, Ministry of Education and Religions, ESPA 2007-2013, Coordinator: Ch. Louis, ITE, Dep. of Biology, University of Crete.

2011 (submitted): “Biochemical and Molecular Studies on the Role of Efflux Transporter Proteins in Fungicide Resistance and Mycotoxin Production by Fusarium Species”, (POSTDOCTORAL RESEACRH PROJECT, Pescticide Laboratory/AUA-Greece, Laboratory of Molecular Phytopathology and Mycotoxin Research/University of Göttingen-Germany, GSRT, European Social Fund and Greek Ministry of Education, Lifelong Learning and Religious Affairs, General Secretariat of Research and Technology).

2011 (submitted): “Fusarium mycotoxins in cereals and by-products”, Framework Program for Research, Technological Development and Innovation 2009-2010 (ΔΙΔΑΚΤΩΡ/0311), Republic of Cyprus, European Union.

Publications of Pesticide Science Laboratory

Doukas EG, Markoglou AN, Vontas JG and Ziogas BN (2012) Characterization of cyp51, mdr and aflR genes and association of DMI resistance phenotypes with fitness and aflatoxin production in Aspergillus parasiticus Speare. Fungal Genetcis and Biology (submitted 29-3-12).

Veloukas T, Markoglou AN and Karaoglanidis GS (2012) Differential effect of SdhB gene mutations of Botrytis cinerea on the sensitivity to SDHI fungicides. Plant Disease (submitted 4-12).

Chronopoulou EG, Papageorgiou AC, Markoglou AN and Labrou NE (2012) Inhibition of human glutathione transferases by pesticides: Development of a simple analytical assay for the quantification of pesticides in water. Journal of Molecular Catalysis B: Enzymatic http://dx.doi.org/10.1016/j.molcatb.2012.04.022

Malandrakis ΑΑ, Markoglou AN and Ziogas BN (2012) PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilia laxa from Greece. Crop Protection 39, 11-17.

Konstantinou S, Karaoglanidis GS, Bardas GA, Minas I, Doukas E and Markoglou AN (2011) Postharvest fruit rots of apple in Greece: Pathogen incidence and relationships between fruit quality parameters, cultivar susceptibility and patulin production. Plant Disease 95, 666-672.

Malandrakis ΑΑ, Markoglou AN, Nikou DC, Vontas JG and Ziogas BN (2011) Molecular diagnostic for detecting the cytochrome b G143S-QoI resistance mutation in Cercospora beticola. Pesticide Biochemistry and Physiology 100, 87-92.

Markoglou AN, Doukas EG and Malandrakis AA (2011) Effect of anilinopyrimidine resistance on aflatoxin production and fitness parameters in Aspergillus parasiticus Speare. International Journal of Food Microbiology 146, 130-136.

Karaoglanidis GS, Markoglou AN, Bardas GA, Doukas EG, Konstantinou S and Kalampokis Ι (2011) Sensitivity of Penicillium expansum field isolates to tebuconazole, iprodione, fludioxonil and cyprodinil and characterization of fitness parameters and patulin production. International Journal of Food Microbiology 145, 195-204.

Malandrakis AA, Markoglou AN and Ziogas BN (2011) Molecular characterization of benzimidazole-resistant Botrytis cinerea field isolates with reduced or enhanced sensitivity to zoxamide and diethofencarb. Pesticide Biochemistry and Physiology 99, 118-124.

Nikologianni A, Andreou P, Nektarios PA and Markoglou AN (2010) Metalaxyl-m leaching from different substrates and drainage systems in intensive green roofs. Acta Horticulturae 881, 725-728.

Stavrianakou S, Liakopoulos G, Miltiadou D, Markoglou AN, Ziogas BN and Karabourniotis G (2010) Antifungal and antibacterial capacity of extracted material from non-grandular and grandular leaf hairs applied at physiological concentrations. Plant Stress 4, 25-30.

Nikou DC, Malandrakis AA, Konstantakaki M, Vontas JG, Markoglou AN and Ziogas BN (2009) Molecular characterization and detection of overexpressed C-14 a-demethylase-based DMI resistance in Cercospora beticola field isolates. Pesticide Biochemistry and Physiology 95(1), 18-27.

Ziogas BN, Nikou DC, Markoglou AN, Malandrakis AA and Vontas JG (2009) Identification of a novel point mutation in the β-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay. European Journal of Plant Pathology 125, 97-107.

Chitzoglou GJ, Athanassiou CG, Markoglou AN and Kavallieratos NG (2008) Influence of commodity on the effect of spinosad dust against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) and Sitophilus oryzae (L.) (Coleoptera: Curculionidae). International Journal of Pest Management 54(4), 277-285.

Markoglou AN, Doukas EG and Ziogas BN (2008) Phenylpyrrole-resistance and aflatoxin production in Aspergillus parasiticus Speare. International Journal of Food Microbiology 127(3), 268-275.

Markoglou AN, Bempelou ED, Liapis KS and Ziogas BN (2007) Determination of benzoylurea residues in tomatoes by high-performance liquid chromatography with UV-diode array and atmospheric pressure chemical ionization-mass spectrometry detection. Journal of AOAC International 90(5), 1395-1401.

Malandrakis AA, Markoglou AN, Nikou DC, Vontas JG and Ziogas BN (2006) Biological and molecular characterization of laboratory mutants of Cercospora beticola resistant to Qo inhibitors. European Journal of Plant Pathology 116, 155-166.

Markoglou AN, Malandrakis AA, Vitoratos AG and Ziogas BN (2006) Characterization of laboratory mutants of Botrytis cinerea resistant to QoI fungicides. European Journal of Plant Pathology 115, 149-162.

Ziogas BN, Markoglou AN, Theodosiou DI, Anagnostou A and Boutopoulou S (2006) A high multi-drug resistance to chemically unrelated oomycetes fungicides in Phytophthora infestans. European Journal of Plant Pathology 115, 283-292.

Ziogas BN, Markoglou AN and Spyropoulou B (2005) Effect of phenylpyrrole-resistance mutations on ecological fitness of Botrytis cinerea and their genetical basis in Ustilago maydis. European Journal of Plant Pathology 113, 83-100.

Ziogas BN, Markoglou AN and Malandrakis AA (2003) Studies on the inherent resistance risk to fenhexamid in Botrytis cinerea. European Journal of Plant Pathology 109, 311-317.

Markoglou AN and Ziogas BN (2002) Genetic control of resistance to the piperidine fungicide piperalin in Ustilago maydis. European Journal of Plant Pathology 108, 21-30.

Ziogas BN, Markoglou AN and Tzima A (2002) A non-Mendelian inheritance of resistance to strobilurin fungicides in Ustilago maydis. Pest Management Science 58, 908-916.

Markoglou AN and Ziogas BN (2002) SBI-fungicides: fungicidal effectiveness and resistance in Botrytis cinerea. Phytopathologia Mediterranea 41, 120-130.

Markoglou AN and Ziogas BN (2001) Genetic control of resistance to the piperidine fungicide fenpropidin in Ustilago maydis. Journal of Phytopathology-Phytopathologische Zeitschrift 149 (9), 551-559.

Markoglou AN and Ziogas BN (2000). Genetic control of resistance to tridemoph in Ustilago maydis. Phytoparasitica 28(4), 349-360.

Markoglou AN and Ziogas BN (1999) Genetic control of resistance to fenpropimorph in Ustilago maydis. Plant Pathology 48, 521-530.