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Dimitrios Georgakopoulos
Associate Professor
2105294355
2105294344
dgeorga@aua.gr

Personal Info

EDUCATION

 

Ph.D. Plant Pathology, University of California, Berkeley, USA,1993

M.Sc. Plant Pathology, Montana State University, USA, 1987

B.Sc. Agricultural University of Athens, GREECE, 1984

SCIENTIFIC INTERESTS

Agricultural Microbiology

Microbial Ecology (Bacterial Ice Nucleation)

Microbial Biosensors

Biological Control of Plant Pathogens

COLLABORATIONS

West Texas A&M University, Department of Life, Earth and Environmental Sciences (USA)

Karlsruhe Institute of Technology, Institute for Meteorology and Climate Research  (Germany)

Environmental Radioactivity Laboratory INRASTES, NCSR “Demokritos”, Athens, Greece

Institute of Life Sciences, Hebrew University of Jerusalem, Israel

Department of Atmospheric Sciences, Colorado State University, USA

Department of Food Science, University of Copenhagen, Denmark

Institut National de la Recherche Agronomique, Avignon, France

Institute of Meteorology and Climate Research, Karlsruhe Institute of Technology, Germany

Montana State University, Department of Plant Sciences and Plant Pathology, Bozeman, Montana, USA

HONORS

Fulbright Scholarship 2008-2009. Visit at the Department of Atmospheric Sciences, Colorado State University and the Department of Plant Sciences and Plant Pathology, Montana State University, USA

Funding for a visit at the Hebrew Univesity of Jerusalem, Israel (November 2014) by the Hellenic Ministry of Education (Bilateral cooperation program)

Affiliate Graduate Faculty member, West Texas A&M University, USA, 2019-

SOCIETIES

Mikrobiokosmos, Hellenic Society for Microbiology

Hellenic Phytopathological Society

CONFERENCE ORGANIZATION

8th Conference of the Hellenic Phytopathological Society, October 1996, Heraklion, Crete, Greece

Workshop «Biological control of Plant Pathogens: from Research to Agricultural Production». Heraklion, Crete, Greece, October 2001

4th Microbiokosmos Conference, Athens, December 2012

Conference «Enzymes in Sustainable Food Production: a Lean Green Approach». June 2013, Chalkidiki, Greece. Final event of the FP7 Marie Curie «LeanGreenFood» Project

XVI ISMPMI Congress (International Society for Molecular Plant Microbe Interactions), Ρόδος, Ιούλιος 2014

6th Microbiokosmos Conference, Athens, April 2015

 

EU-LIFE 2020/CLIMATE ACTION (CCA/GR/001747) "Forecasting and protecting fruit crops from frost damage" (FROSTDEFEND). 2021-2025. Scientific Coordinator for the AUA. Coordinator: NCSR-Demokritos (Athens, Greece). Total budget € 1941770. AUA budget € 386685
PENED 2003 : Biochemical and genetic characterization of bacterial antagonists of phytopathogenic fungi and their incorporation in sugar beet seed pellets. Total budget 126000 euros. Principal Investigator. National Funding.
Greece-France 2003-06: Les sources et quantités de noyaux glacogènes de l'origine bactérienne dans l'atmosphère et leur rôle dans les processus atmosphériques (Sources and quantity of bacterial ice nuclei in the atmosphere and their role in atmospheric processes). Total budget 24210 euros. Principal Investigator. French partner : Dr. Cindy E. Morris, INRA-Avignon. National Funding.
Pythagoras II 2005 : Development and application of whole cell bioluminescent biosensors for toxicity evaluation. Total budget 90000 euros (30000 euros for the Laboratory of General and Agricultural Microbiology). Partner to the Laboratory of Chemistry, AUA.  National Funding.
LeanGreenFood 2009-2013. (EU-7th Framework Programme-People-Initial Training Network). Total budget (AUA) 629000 euros. Partner. EU Funding.

UPDATEST-Training professionals and students on new testing technologies for the food sector (EU-Erasmus+ Cooperation for innovation and the exchange of good practices). 2016-2018. Partner. Coordinator for the AUA: Laboratory of Chemistry (Prof. Kostas Georgiou). Budget for the AUA: 49160 EUR.
Cost Action ADOPT (CA18226) "New approaches in detection of pathogens and aeroallergens" 2019-2023. Management Committee Member for Greece.

EU LIFE FROSTDEFEND (2021-2025) "Forecasting and protecting fruit crops from frost damage". Coordinator: National Center for Scientific Research "Demokritos". Coordinator for AUA. Program budget € 1972230. AUA budget: € 386685.

PhD Thesis: “Analysis of expression of a phenazine antibiotic biosynthesis locus in Pseudomonas aureofaciens PGS12 in vitro and on seeds with the ice nucleation reporter gene”. University of California, Berkeley, USA, 1993

Master of Science Thesis: “Epidemiology of Pseudomonas syringae on barley”. Montana State University, USA, 1987

Full Papers in International Refereed Journals

Reprints are available here: https://www.researchgate.net/profile/Dimitrios-Georgakopoulos-5

Naruki Hiranuma, Brent W. Auvermann, Franco Belosi, Jack Bush, Kimberly M. Cory, Dimitrios G. Georgakopoulos, Kristina Höhler, Yidi Hou, Larissa Lacher, Harald Saathoff, Gianni Santachiara, Xiaoli Shen, Isabelle Steinke, Romy Ullrich, Nsikanabasi S. Umo, Hemanth S. K. Vepuri, Franziska Vogel, and Ottmar Möhler (2021) Laboratory and field studies of ice-nucleating particles from open-lot livestock facilities in Texas. Atmospheric Chemistry and Physics 21 (18) pp.14215-14234,  https://doi.org/10.5194/acp-21-14215-2021

Vepuri, H. S. K., Rodriguez, C. A., Georgakopoulos, D. G., Hume, D., Webb, J., Mayer, G. D., and Hiranuma, N.: Ice-nucleating particles in precipitation samples from the Texas Panhandle, Atmos. Chem. Phys., 21, 4503–4520, https://doi.org/10.5194/acp-21-4503-2021, 2021.

Tsivou, M.,  Giannadaki, E.,  Hooghe, F.,  Roels, K.,  Van Gansbeke, W.,  Garribba, F.,  Lyris, E.,  Deventer, K.,  Mazzarino, M.,  Donati, F.,  Georgakopoulos, D.G.,  Van Eenoo, P.,  Georgakopoulos, C.G.,  de la Torre, X.,  Botrè, F. (2017) Doping control container for urine stabilization: A pilot study. Drug Testing and Analysis 9(5), pp. 699-712
 
Venieraki, A.,  Tsalgatidou, P.Ch.,  Georgakopoulos, D.G.,  Dimou, M.,  Katinakis, P. (2016) Swarming motility in plant-associated bacteria. Hellenic Plant Protection Journal 9(1): 16-27
 
Komaitis, E., Vasiliou, E., Georgakopoulos, D., Georgiou, C. (2015) Fully automated fluidic analyzer for food quality assessment using bioluminescent biosensors. In:  Bioluminescent Microbial Biosensors: Design, Construction, and Implementation, 5, pp. 1-14
 
Vaxevanidou, K.,  Christou, C.,  Kremmydas, G.F.,  Georgakopoulos, D.G.,  Papassiopi, N. (2015) Role of indigenous arsenate and IRON(III) respiring microorganisms in controlling the mobilization of arsenic in a contaminated soil sample. Bulletin of Environmental Contamination and Toxicology 94(3): 282-288  
 
Thomas C. J. Hill, Bruce F. Moffett, Paul J. DeMott, Dimitrios G. Georgakopoulos, William L. Stump, Gary D. Franc (2014) Ice nucleation active bacteria on plants and in precipitation measured using quantitative PCR. Applied and Environmental Microbiology 80:1256-1267
 
Lukasiak J, Olsen K, Georgiou CA, Georgakopoulos DG. (2014). Bacterial reporter strains for d‑xylose content analysis in arabinoxylans. European Food Research and Technology 238: 275-283
 
Constantinos Georgiou, Lucyna Lekawska-Andrinopoulou, Efstathios G. Vasiliou, Dimitrios G. Georgakopoulos and Costas P. Yialouris (2013). Rapid enzymatic method for pectin methyl esters determination. Journal of Analytical Methods in Chemistry Article Number: UNSP 854763
 
Kremmydas GF, Tampakaki AP, Georgakopoulos DG (2013). Characterization of the biocontrol activity of Pseudomonas fluorescens strain X reveals novel genes regulated by glucose. PLoS One 8(4) 10.1371/journal.pone.0061808
 
Lukasiak J, Olsen K, Georgiou CA, Georgakopoulos DG. (2013). Bioluminescence and ice nucleation microbial biosensors for L-arabinose content analysis in arabinoxylans. European Food Research and Technology 237(3), 291-298
 
Lukasiak J, Georgiou CA, Olsen K, Georgakopoulos DG. (2012). Development of an l-rhamnose bioluminescent microbial biosensor for analysis of food ingredients. European Food Research and Technology 235(3), 573-579
 
Fotiadis CT, Dimou M, Georgakopoulos DG, Katinakis P, Tampakaki AP. (2012). Functional characterization of NopT1 and NopT2, two type III effectors of bradyrhizobium japonicum. FEMS Microbiology Letters 327(1), 66-77
 
Paul J. DeMott, Ottmar Möhler, Olaf Stetzer, Gabor Vali, Zev Levin, Markus D., Petters, Masataka Murakami, Thomas Leisner, Ulrich Bundke, Holger Klein, Zamin A. Kanji, Richard Cotton, Hazel Jones, Stefan Benz, Maren Brinkmann, Daniel Rzesanke, Harald Saathoff, Mathieu Nicolet, Atsushi Saito, Bjorn Nillius, Heinz Bingemer, Jonathan Abbatt, Karin Ardon, Eli Ganor, Dimitrios G. Georgakopoulos, Clive Saunders. (2011). Resurgence in Ice Nuclei Measurement Research. Bulletin of the American Meteorological Society 92 (12), 1623-1635
 
Maria Tsivou, Dimitrios G. Georgakopoulos, Helen A. Dimopoulou, Michael Α. Koupparis and Julia Atta-Politou, et al. (2011). Stabilization of human urine doping control samples: a current opinion. Analytical and Bioanalytical Chemistry 401(2), 553-561
 
Komaitis, E., Vasiliou, E., Kremmydas, G., Georgakopoulos, D. G., & Georgiou, C. (2010). Development of a fully automated flow injection analyzer implementing bioluminescent biosensors for water toxicity assessment. Sensors10(8), 7089-7098
 
Maria Tsivou, Helen A. Dimopoulou, Dimitris G. Georgakopoulos, Michael Α. Koupparis, Julia Atta-Politou and Costas G. Georgakopoulos. (2010). Stabilization of human urine doping control samples: IV. Human chorionic gonadotropin. Analytical and Bioanalytical Chemistry 398(3), 1313-1318
 
Tsivou, M., Dimopoulou, H. A., Leontiou, I., Georgakopoulos, D. G., Koupparis, M., Atta-Politou, J., et al. (2010). Stabilization of human urine doping control samples: III. recombinant human erythropoietin. Clinica Chimica Acta, 411(5-6), 448-452
 
Georgakopoulos, D. G., Després, V., Fröhlich-Nowoisky, J., Psenner, R., Ariya, P. A., Pósfai, M., et al. (2009). Microbiology and atmospheric processes: Biological, physical and chemical characterization of aerosol particles. Biogeosciences, 6(4), 721-737
 
Tsivou, M., Livadara, D., Georgakopoulos, D. G., Koupparis, M. A., Atta-Politou, J., & Georgakopoulos, C. G. (2009). Stabilization of human urine doping control samples. Analytical Biochemistry, 388(2), 179-191
 
Tsivou, M., Livadara, D., Georgakopoulos, D. G., Koupparis, M. A., Atta-Politou, J., & Georgakopoulos, C. G. (2009). Stabilization of human urine doping control samples: II. microbial degradation of steroids. Analytical Biochemistry, 388(1), 146-154
 
Möhler, O., Georgakopoulos, D. G., Morris, C. E., Benz, S., Ebert, V., Hunsmann, S., et al. (2008). Heterogeneous ice nucleation activity of bacteria: New laboratory experiments at simulated cloud conditions. Biogeosciences, 5(5), 1425-1435
 
Morris, C. E., Georgakopoulos, D. G., & Sands, D. C. (2004). Ice nucleation active bacteria and their potential role in precipitation. Journal De Physique. IV 121, 87-103
 
Georgakopoulos, D. G., Fiddaman, P., Leifert, C., & Malathrakis, N. E. (2002). Biological control of cucumber and sugar beet damping-off caused by Pythium ultimum with bacterial and fungal antagonists. Journal of Applied Microbiology 92(6), 1078-1086
 
Ververidis, P., Davrazou, F., Diallinas, G., Georgakopoulos, D., Kanellis, A. K., & Panopoulos, N. (2001). A novel putative reductase (Cpd1p) and the multidrug exporter Snq2p are involved in resistance to cercosporin and other singlet oxygen-generating photosensitizers in Saccharomyces cerevisiae. Current Genetics 39(3), 127-136
 
Georgakopoulos, D. G., Hendson, M., Panopoulos, N. J., & Schroth, M. N. (1994). Analysis of expression of a phenazine biosynthesis locus of Pseudomonas aureofaciens PGS12 on seeds with a mutant carrying a phenazine biosynthesis locus-ice nucleation reporter gene fusion. Applied and Environmental Microbiology 60(12), 4573-4579
 
Georgakopoulos, D. G., Hendson, M., Panopoulos, N. J., & Schroth, M. N. (1994). Cloning of a phenazine biosynthetic locus of Pseudomonas aureofaciens PGS12 and analysis of its expression in vitro with the ice nucleation reporter gene. Applied and Environmental Microbiology 60(8), 2931-2938
 
Georgakopoulos, D. G., & Sands, D. C. (1992). Epiphytic populations of Pseudomonas syringae on barley. Canadian Journal of Microbiology 38(2), 111-114
 
Caristi, J., Sands, D. C., & Georgakopoulos, D. G. (1991). Simulation of epiphytic bacterial growth under field conditions. Simulation 56(5), 295-301
 

Book Chapters

Komaitis, E., Vasiliou, E., Georgakopoulos, D., Georgiou, C. (2015) Fully automated fluidic analyzer for food quality assessment using bioluminescent biosensors. In:  Bioluminescent Microbial Biosensors: Design, Construction, and Implementation, 5, pp. 1-14

Malathrakis, Ν. Ε. and D. G. Georgakopoulos. 2001. Plant Diseases and Plant Ecology, pp 183-203, In: Advances in Agroecology, CRC Press, Boca Raton, Η.Π.Α.

Conference presentations (Full Papers in Conference Proceedings, Oral and Poster Presentations included in Book of Abstracts)

1.     Venieraki Α., Tsalgatidou P., Georgakopoulos D., Antoniou P.P., Dimou Μ. και P. Katinakis. 2014. Modes of action of Pseudomonas antagonists as biocontrol agents against plant fungal pathogens. 17th Conference of the Hellenic Phytopathological Society, Volos, Greece.

2.     Łukasiak, C.A. Georgiou, K. Olsen and D.G. Georgakopoulos. 2013. Bioluminescence and ice nucleation microbial biosensors for monosaccharide content analysis in arabinoxylans. Eurofoodchem XVII Conference, Istanbul, Turkey.

3.     Tsivou M, Giannadaki E, Georgakopoulos DG et al. 2013. Stabilization in urine collection containers: reality or science-fiction? 31st Cologne Workshop on Dope Analysis. Cologne, Germany. In W. Schänzer, M. Thevis, H. Geyer, U. Mareck (Eds.), Recent Advances in Doping Analysis, vol. 21, Sport & Buch Strauß, Köln, Germany, 2013, pp.242-246.
  1. K. Vaxevanidou, C. Christou, D. Georgakopoulos and N. Papassiopi. 2013. Mobilization of Arsenic from a contaminated soil sample by Arsenate and Fe(III) reducing microorganisms. 4th International CEMEPE & SECOTOX Conference,  Mykonos, Greece.
  1. Lukasiak J., C.A. Georgiou, K. Olsen and D.G. Georgakopoulos. 2012. Microbial biosensors for monosaccharide content analysis in arabinoxylans. 5th Microbiokosmos conference, Athens, Greece.
  1. Kremmydas G. and Georgakopoulos D.G. 2011. Genetic characterization of the antagonism against phytopathogenic fungi by Pseudomonas fluorescens X. 4th Microbiokosmos conference, Ioannina, Greece.
  1. Lukasiak J., Georgiou C.A., Olsen Κ., Georgakopoulos D.G. 2011. Development of bioluminescent microbial biosensors to monitor enzymatic processing of food ingredients. 4th Mikrobiokosmos conference, Ioannina, Greece.
  1. D. G. Georgakopoulos, C. A. Georgiou, A. Janssen, J. Mikkelsen, K. Grunnert, and K. Olsen. 2010. LeanGreenFood: A Marie Curie FP7 Initial Training Network in novel enzymatic processing of food ingredients. Poster Presentation, 3rd Microbiokosmos Conference, Thessaloniki, Greece.
  1. P. J. DeMott, A. J. Prenni, D. G. Georgakopoulos, G. D. Franc, R. C. Sullivan, S. M. Kreidenweis. 2009. Studies of biological aerosols as ice nuclei: Existing constraints and new measurements (Invited). American Geophysical Union (AGU) 2009 Fall Meeting, San Francisco, CA, USA.
  1. D.G. Georgakopoulos 2009. Microbes and climate. 2nd Mikrobiokosmos conference, Athens, Greece.
  1.  D. G. Georgakopoulos, A. J. Prenni, S. M. Kreidenweis and P. J. DeMott. 2009. Use of a Continuous Flow Diffusion Chamber for characterization of ice nuclei in bioaerosols. In Nucleation and Atmospheric Aerosols, 18th International Conf., Institute of Chemical Process Fundamentals ASCR and Czech Aerosol Society, 277-280.
  1. M.Tsivou, D.G. Georgakopoulos, M.A. Koupparis, J. Atta-Politou, D. Livadara, C.G. Georgakopoulos, Manfred Donike. 2009. Stabilization of endogenous steroids in sport urine samples Workshop, 27th Cologne Workshop on Dope Analysis, Cologne, Germany. In W. Schänzer, H. Geyer, A. Gotzmann, U. Mareck (Eds.), Recent Advances in Doping Analysis, vol. 17, Sport & Buch Strauß, Köln, Germany, 2009, pp. 93-94.
  1. G. Kremmydas, N. Delimpaltadakis and D. G. Georgakopoulos. 2008. Genetic characterization of the antagonism against phytopathogenic fungi and Oomycetes in the bacterium Pseudomonas fluorescens X. 14th Conference of the Hellenic Phytopathological Society, Nafplio, Greece.
  1. N. Salari, A.N. Markoglou and D.G. Georgakopoulos. 2008. Characterization of antimicrobial metabolites produced by the bacterium Pseudomonas fluorescens X. 1st Mikrobiokosmos conference, Athens, Greece.
  1. N. Salari, A.N. Markoglou and D.G. Georgakopoulos. 2008. Characterization of antimicrobial metabolites produced by the bacterium Pseudomonas fluorescens X. 14th Conference of the Hellenic Phytopathological Society, Nafplio, Greece.
  1. M. Tsivou, D.G. Georgakopoulos, M. Koupparis, J. Atta-Politou, D. Livadara, C.G. Georgakopoulos, Manfred Donike. 2008. Preservation of urine doping control samples: preliminary results. 26th Cologne Workshop on Dope Analysis, Cologne, Germany. In W. Schänzer, H. Geyer, A. Gotzmann, U. Mareck (Eds.), Recent Advances in Doping Analysis, vol. 16, Sport & Buch Strauß, Köln, Germany, 2008, pp. 41-50.
  1. D. Sands, C. Morris and D. Georgakopoulos. 2007. Is there a role for ice nucleation activity in bacterial dissemination? Keynote paper, Symposium on "Biological Ice Nucleators in the Atmosphere - at the Crossroads of Physics and Biology", IUGG XXIV General Assembly, Perugia, Italy.
  1. O. Moehler, C. Morris and D. Georgakopoulos. 2007. New cloud chamber studies on the ice nucleation activity of bacteria in the atmosphere. Symposium on "Biological Ice Nucleators in the Atmosphere - at the Crossroads of Physics and Biology", IUGG XXIV General Assembly, Perugia, Italy.
  1. C.A. Georgiou, E.M. Komaitis, E.G. Vasiliou, G. Kremydas, D.G. Georgakopoulos. 2007. Response of Vibrio fischeri Whole Cell Biosensors to Olive Oil Phenolics: Monitoring Early Oxidation Events during Frying”. Poster Presentation, 5th Euro Fed Lipid Congress, Gothenburg, Sweden.
  1. E.M. Komaitis, E.G. Vasiliou, D.G. Georgakopoulos, C.A. Georgiou. 2007. Assessment of Oil Quality during Frying through V. fischeri Whole Cell Biosensors. 2nd Hellenic Conference on Fats, Oils, Lipids: Present and Future. Athens, Greece.
  1. E. Komaitis, E. Vasiliou, G. Kremmydas, D. Georgakopoulos, C. Georgiou. 2007. Response of Vibrio fischeri Whole Cell Biosensors to Olive Oil Phenolics, Food Technology and Biotechnology, Athens, Greece.
  1. A. Markoglou, G. Kremmydas, S. Arnaouteli, N. Salari and D. G. Georgakopoulos. 2006. Isolation and qualitative determination of an antimicrobial metabolite produced by Pseudomonas fluorescens. 12th Conference of the Mediterannean Phytopathological Union, Rhodes, Greece. Pages?
  1. E. Komaitis, D. Georgakopoulos and C. Georgiou. 2006. Flow technologies for automation of luminescence whole-cell biosensors: water toxicity assessment. 5th Aegean Analytical Chemistry Days, Thessaloniki, Greece.
  1. Sands, D.C., C. E. Morris, D. G. Georgakopoulos and J. Heimbach. 2005. Bacterial ice nuclei from plant canopies: What impact on atmospheric load of ice nuclei and on atmospheric processes? Weather Modification Association Annual Meeting, Rockville, MD, USA.
  1. Georgakopoulos, D.G., R. Tilcher, U. Fischer, F. Ioannidis, K. Doulias and N. E. Malathrakis. 2002. Biological and integrated control of sugar beet damping-off in the field. 11th National Conference of the Hellenic Phytopathological Society, 1-4 October 2002, Preveza, Greece.
  1. Georgakopoulos, D.G. and N. E. Malathrakis. 2002. Integrated control of cucumber damping-off with bacteria formulated in peat and low rates of fungicides. 11th National Conference of the Hellenic Phytopathological Society, 1-4 October 2002, Preveza, Greece.
  1. Georgakopoulos, D.G., P. Fiddaman, C. Leifert and N. E. Malathrakis. 2000. Evaluation of antagonistic bacteria and fungi for biological control of sugar beet and cucumber damping-off caused by Pythium ultimum. 6th IOBC/WPRS-EFPP Biocontrol Workshop, 30 November-3 December 2000, Sevilla, Spain.
  1. Georgakopoulos, D.G., P. Fiddaman, C. Leifert and N. E. Malathrakis. 2000. Biological control of sugar beet and cucumber damping-off with bacteria and fungi. 10th National Conference of the Hellenic Phytopathological Society, 3-5 October 2000, Kalamata, Greece.
  1. Ververidis P., G. Diallinas, D. G. Georgakopoulos, Α. Κ. Κanellis and Ν. Panopoulos. 1996. A multidrug resistance gene product is involved in protection of Saccharomyces cereviciae from the toxin Cercosporin. 8th National Conference of the Hellenic Phytopathological Society, 22-24 October 1996, Heraklion, Crete, Greece.
  1. Georgakopoulos, D. G., M. Hendson and M. N. Schroth. 1994. Analysis of expression of a phenazine biosynthetic locus from Pseudomonas aureofaciens PGS12 with the ice nucleation reporter gene. Ryder, M. H., P. rhizosphere bacteria. Third International Workshop on Plant Growth- Promoting Rhizobacteria, Adelaide, South Australia, Australia, March 7-11,1994. 288p. CSIRO Publications: East Melbourne, Victoria, Australia, pp 110-113.
  1. Georgakopoulos, D. G., M. Hendson and M. N. Schroth. 1993. Analysis of expression of a phenazine antibiotic DNA locus in culture and on seeds with the ice nucleation reporter gene system. Phytopathology 83 (6): 691.
  1. Georgakopoulos, D. G., D. C. Sands and A. L. Scharen.1987. Epiphytic Pseudomonas syringae on Barley: A Quantitative Study. Phytopathology 77 (12): 1725-1726.

 

 

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